Vetnews | November 2025 18 « BACK TO CONTENTS Article Ginseng (30 days) The Capsule of testis was formed by dense Collagen fibers, there was many Spermatogenic cells surrounded by collagen bundle, Great Cavities of seminiferous tubule had degenerated spermatogonia and other cells of Spermatocytes were absent homogenized odema were present inside the lumen of Certain seminiferous tubules (fig: 5). Ibuprofen (10 days) and ginseng (20 days) The testicular tissue was coated with a capsule of collagen bundles, the parenchyma of the testis was occupied with crowded seminiferous tubules, which had different stages of Spermatogenic development, the interstitial connective tissue had groups of Leydig cells with blood capillaries (Fig. 6). Ibuprofen + ginseng together (30 Days) Seminiferous tubules contained the different stages of spermatogenic development. Primary spermatocytes in certain tubules appeared atrophied, and secondary spermatocytes were abundant and intermingled with spermatids. Scanty spermatzoa were seen in certain seminiferous tubules. The blood vessels in the interstitial connective tissue were engorged with hemolyzed blood in the form of pools with the presence of small fat droplets in the lumen of the basement cavity (Fig 7). The histological analysis of rats given ibuprofen and dimethyl sulfoxide for 10 and 30 days showed various histological alterations, such as hemolyzed blood vessels, fewer spermatogonia, and a lower sperm count. Seminiferous tubules were found to contain the various phases of spermatogenic development, and the majority of spermatogenic cells were improved when ginseng extract was used to cure and prevent the negative effects of ibuprofen. Ibuprofen has been shown in several studies to have a remarkable dual effect on both Leydig and Sertoli cells. This makes it the NSAID with the most extensive endocrine-disturbing qualities found in males to date, considering all chemical classes. Only on Leydig cells have prior ex vivo investigations on the adult testis indicated antiandrogenicity (32,33,34). The study found that the interstitium widened with a large gap between cut parts of the seminiferous, which was in agreement with the findings of Halawa and Nagwa (35). Numerous seminiferous tubules displayed apparent hypocellularity at every stage, along with symptoms of nuclear pyknosis, cell degeneration, and loss of germ cells. In several areas of the section, cell membranes were visible, although seminiferous tubular epithelium appeared to be separated from the basement membrane. Hyaline material was deposited in the interstium, and numerous dilated, clogged blood vessels were visible. On the other hand, according to the current study, ginseng root extract effectively improves and shields testicular tissue from the harmful effects of ibuprofen. Ginseng has been demonstrated in subsequent research to enhance the number of sperm in both people and animals. Rats given ginseng have shown an enhanced rate of spermatogenesis by activation of the testicular cAMP-responsive element modulator (CREM) (36) and elevation of glial cell-derived neurotrophic factor (GDNF) production in Sertoli cells (37). Ginsenosides (0.01 mg/ml) have also been demonstrated to promote sperm development in vitro, according to Chen et al. (38). In the other study, it was discovered that via modifying the expression of Fas/Fas-L, Korean red ginseng (300 mg/kg) taken every other day for four weeks might prevent rats from experiencing spermatogenesis impairment caused by zearalenone (39). Ameliorative Potential of Alcoholic Ginseng Extract .... <<< 17 Figure 5: Histological section from the testis of the rat (G5) showed Capsule of testis formed by Collagen bundle (A).(H & E X10) Figure 6: Histological section from the testis of the rat (G6) showed parenchyma of testis, Crowding of seminiferous tubules, with different stages of spermatogenic development (A). Interstitial connective tissue with blood Capillaries (B) Leydig cells (C) (H&E x10) Figure 7: Histological section from the testis of the rat (G7) showed Seminiferous tubules, spermatogenic development (A). hemolyzed blood with small droplets of fat (B). Atrophy of primary spermatocytes (C). Hypertrophy of secondary Spermatocyte (D) (H&E X 40)
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