Vetnuus | July 2024 25 Events I WVAC 2024 of feed) and group IV (probiotic + ascorbic acid at 1 g/kg and 200 mg/kg of feed, respectively). The treatments were administered via feed for 35 days (D1 to D35). The 8-OHdG gene was significantly lower in the probiotic-administered group. The expression level of HSP70 was lowest in the ascorbic acid group while IL-10 level of expression was highest in the probiotic + ascorbic acid group. The administered antioxidants were efficient in exhibiting anti-stress effects at the level of gene expression. We conclude that probiotic, ascorbic acid and probiotic + ascorbic acid reduced oxidative gene damage, reduced the expression of heat shock protein 70 and increased the level of IL-10 gene respectively, in broiler chickens exposed to heat stress. v Epidemiological study of Campylobacter jejuni isolated from gastroenteritis patients in Tokyo Japan Satoru Akase Tokyo Metropolitan Institute of Public Health, Tokyo, Japan In Japan, food poisoning by Campylobacter jejuni has been the highest number of bacterial causative cases for two decades. Most of all incidents were caused by raw or undercooked chicken meat and offal. Penner serotyping method for C. jejuni has been used as epidemiological analysis for food poisoning investigations, but the typing rate was less than 50%, making it difficult to clarify the route of contamination. Moreover, MLST (multi-locus sequence typing) is used as a representative typing method worldwide, but it costs much to analysis and makes difficult as daily typing of C. jejuni for local governments. The Penner PCR capsule typing method has been developed recently, which allows us to resolve both the problems of typing rate and cost. In this study, we conducted those three typing methods for C. jejuni isolates in Tokyo. As a result, we would like to report a significantly improvement in typing rate by the simple typing method. Materials and Methods: 1) Sample strains: 120 of C. jejuni clinical isolates in Tokyo. 2) Analysis method: Penner serotyping method1), Penner PCR capsule typing method (Heat-Stable type)2) and MLST (Sequence type, Clonal complex)3). Results and Discussions: The typing rate for each method was 43.3% (52/120) for Serotyping, 95.0% (114/120) for PCR capsule typing and 100% (120/120) for MLST. C. jejuni types most often isolated from patients in Tokyo were serotypes (group O, group D, group B), HS types (HS2, HS4complex, HS19), ST (ST22, ST918, ST50, ST4526), and CC (CC21, CC22, CC48). Our study clarified that the typing rate of the Penner PCR capsule typing method was sufficiently high, moreover, the typing cost was lowest among the three methods, and it was also suitable for daily typing of C. jejuni in local governments. We are currently using the Penner PCR capsule typing method for epidemiological investigations of food poisoning in Tokyo. Molecular and serological prevalence of corridor disease (buffalo associated Theileria parva infection) in cattle populations at the livestock/game interface of KwaZulu-Natal province, South Africa Sikhumbuzo Mbizeni University of South Africa & Department of Agriculture and Animal Health, Johannesburg, South Africa. The increase in reports of Corridor disease (CD) outbreaks in recent years has raised questions about the probability of adaptation of buffaloderived Theileria parva strains in cattle herds adjacent to game reserves. A cross-sectional study was conducted from March 2016 to December 2018 to investigate the extent of the occurrence of T. parva infections in cattle in the CD-controlled area of KwaZulu-Natal province. Blood samples were collected from 1137 cattle from 14 herds and analysed by quantitative real-time PCR (qPCR) and indirect fluorescent antibody test (IFAT) to determine the prevalence of T. parva. A total of 484 samples from 4 of the 14 herds were further tested on qPCR for the presence of T. taurotragi infections. Data was analysed using descriptive statistics and a chi-square test was used to assess association between variables. The overall prevalence of T. parva was 1.3% (95%CI:1-2%) and 19.9% (95% CI:17-22%) on qPCR and IFAT, respectively. The qPCR-positive samples were detected in March and May and IFAT-positive samples were detected in all seasons sampled, with higher numbers during summer months. The chi-square test showed that T. parva prevalence rates based on both qPCR and IFAT were positively associated with herds with previous history of CD outbreaks (χ2 = 8.594, P = 0.003; 69.513, P < 0.001, respectively). The overall prevalence of T. taurotragi was 39.4% (95% CI: 35-44%) with the herd-level prevalence ranging between 35.0% and 43.4%. Results from this study demonstrate the extent of occurrence of subclinical carriers of T. parva infections in cattle populations at a livestock/game interface area of KwaZulu-Natal province. The molecular and seroprevalence rates were low when compared with areas where cattle-adapted T. parva infections are endemic. The adaptation of buffaloderived T. parva in cattle population resulting in cattle-cattle transmissions seems to be unlikely under the current epidemiological state. 24-Hour Toll-Free Helpline: 0800 21 21 21 >>> 26
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